Regulation of IP-10 mRNA by CsA, FK506, and rapamycin.

IP-10 mRNA levels were assessed by RT-PCR in human PBLs. Human PBLs were stimulated for 8 hours with ethanol diluent, PMA, Iono, PMA+Iono, or α–CD3/28+PMA in the absence or presence of CsA, FK506, or rapamycin (Rap). RT-PCR, using IP-10– and β-actin–specific primers, was carried out as outlined in “Materials and methods.” (A) Results of 1 experiment representative of 2 performed using human PBLs from different human donors are shown. (B) IP-10 bands were quantitated, and mean values ± SEMs of 2 experiments normalized to β-actin mRNA levels using ImageQuant software are graphically represented. Ionomycin-driven attenuation of IP10 mRNA by CsA and FK506, but not that of rapamycin, was significant (P < .05).