Increased Ltn, IL-8, and MIP-1β total protein levels in stimulated human T lymphocytes and inhibition by CsA.

Human PBLs were treated in the presence and absence of PMA (10 ng/mL) and PMA+Iono (1 μM) for 12 hours in the presence or absence of CsA (1 μM) as indicated. Cells were permeabilized, fixed, and labeled with PE-conjugated anti–human (A) Ltn mAb, (B) IL-8 mAb, and (C) MIP-1β mAb to measure chemokine protein expression by immunofluorescence. In each column labeled PMA and PMA+Ionomycin, unstimulated cells (dotted line/unshaded) are compared to PMA- and PMA+Ionomycin–treated (solid line/shaded) samples. Adjacent columns examine the effects of CsA on protein expression. Stimulated (PMA or PMA+Iono) cells (dotted line/unshaded) are compared to CsA-pretreated (solid line/shaded) samples.