Effect of plasmid DNA immunizations with proinflammatory chemokine fusion constructs with gp120 or gp140-14.

Plasmid DNA immunizations with proinflammatory chemokine fusion constructs with gp120 or gp140-14 inhibit pseudotype HIV-1 entry. Pooled sera (at various dilutions, shown) from mice immunized with gp120 fused with MCP-3 or MDCs (pMCP3gp120 and pMDCgp120, respectively) were compared with the pooled sera from mice immunized with gp140-14 fused with MDCs and vMIP2 (pMDCgp140-14 and pvMIP2gp140-14, respectively) for their ability to inhibit entry of pseudotype HIV virus expressing Envs from various isolates, such as 89.6 (for R5×4) (panel A), NL4-3 (for X4) (panel B), and JRFL (for R5) (panel C), respectively. Luciferase gene assay (luciferase activity) is described in “Materials and methods.”22Control sera were from mice immunized with MCP-3 fusion construct with EGFP (pMCP3-EGFP), or from PBS-treated mice. P indicates comparison with pMCP3-EGFP. *P indicates comparisons between pMDCgp140-14 and pMCP3gp120. **P indicates comparisons between pMDCgp140-14 and pMDCgp120.