Fig. 1.
Anti-LacCer antibody induced superoxide generation by neutrophils but not DMSO-treated HL-60 cells.
Neutrophils and DMSO-treated HL-60 cells were incubated at 37°C for 30 minutes in 96-well plates coated with anti-LacCer IgM T5A7, anti-LacCer IgM Huly-m13, normal IgM, anti-GM3 IgG3 DH2, or normal IgG3. As a positive control of superoxide generation, cells were incubated with 10−7 M fMLP or 0.5% DMSO (as a solvent control) in bovine serum albumin–coated wells at 37°C for 30 minutes. Superoxide production was calculated by measurement of the superoxide dismutase–inhibitable reduction of cytochrome c at 550 nm. Each bar shows the mean ± SD of 4 independent experiments.
