Fig. 2.
Expression of LacCer on neutrophils and HL-60 cells.
Neutrophils and undifferentiated and DMSO-treated HL-60 cells were incubated with anti-LacCer IgM T5A7 or normal IgM for 30 minutes at 4°C. After washing with PBS, cells were stained with fluorescein isothiocyanate–labeled rabbit antimouse IgM antibody and analyzed by flow cytometry.