American Society of Hematology

Fig. 4.

$Fig. 4. Two-dimensional HPTLC analysis of lipid components in DIM fractions of neutrophils, undifferentiated, and DMSO-treated HL-60 cells. / Lipid compositions of neutrophils, undifferentiated, and DMSO-treated HL-60 cells were analyzed by 2-dimensional HPTLC, and 10-cm thin-layer chromatography (TLC) plates were sprayed with primulin. 1st, 2nd, and 3rd arrows indicate the directions of the first, second, and third chromatographic runs, respectively. Lysate indicates whole cell lysates of neutrophils; DIM, fraction 5; anti-LacCer IgM, immunoprecipitated fraction of DIM with anti-LacCer Huly-m13; standards, standard lipids; PE, phosphatidylethanolamine; PS, phosphatidylserine; PC, phosphatidylcholine; GlcCer, glucosylceramide; Cer, ceramide; SM, sphingomyelin; Chol, cholesterol; GM3, NeuAc3Ga14Glc1Cer; GD1a, IV3NeuNAcII3NeuNAcGgOse4Cer.$

Two-dimensional HPTLC analysis of lipid components in DIM fractions of neutrophils, undifferentiated, and DMSO-treated HL-60 cells.

Lipid compositions of neutrophils, undifferentiated, and DMSO-treated HL-60 cells were analyzed by 2-dimensional HPTLC, and 10-cm thin-layer chromatography (TLC) plates were sprayed with primulin. 1st, 2nd, and 3rd arrows indicate the directions of the first, second, and third chromatographic runs, respectively. Lysate indicates whole cell lysates of neutrophils; DIM, fraction 5; anti-LacCer IgM, immunoprecipitated fraction of DIM with anti-LacCer Huly-m13; standards, standard lipids; PE, phosphatidylethanolamine; PS, phosphatidylserine; PC, phosphatidylcholine; GlcCer, glucosylceramide; Cer, ceramide; SM, sphingomyelin; Chol, cholesterol; GM3, NeuAc3Ga14Glc1Cer; GD1a, IV³NeuNAcII³NeuNAcGgOse₄Cer.

This Feature Is Available To Subscribers Only