Effect of PP1, wortmannin, SB203580, H7, and PD98059 on T5A7-induced neutrophil superoxide generation.

Neutrophils were preincubated for 30 minutes in the presence of 0.0005 to 200 μM PP1(●), wortmannin(○), SB203580(▪), H7(▵), or PD98059(▴) at 4°C and then incubated in anti-LacCer IgM–coated 96-well plates at 37°C for 30 minutes. Neutrophils were also incubated in anti-LacCer IgM–coated wells (solid bar) or in normal IgM–coated wells (open bar) in the absence of inhibitors. Data show the mean ± SD of 4 independent experiments.