Fig. 9.
Fig. 9. T5A7-induced p38 MAPK activation in neutrophils. / (A) Neutrophils were incubated in anti-LacCer IgM T5A7–coated (anti-LacCer IgM) or normal IgM–coated (normal IgM) 10 cm dishes at 37°C for indicated periods. After incubation, lysates were prepared and immunoprecipitated by rabbit anti–p38 MAPK IgG, followed by SDS-PAGE and electroblotting. The membranes were probed with mouse anti–pp38 MAPK IgM (pp38 MAPK). Further, membranes were reprobed with rabbit anti–p38 MAPK IgG (p38 MAPK). (B) Phosphorylation of p38 MAPK was calculated by scanning the signals of phosphorylated and recovered p38 MAPK by Scion image. The activity of p38 MAPK is expressed as fold increase compared with that of cells kept on ice (0 minute). Each point shows the mean ± SD of independent 3 experiments.

T5A7-induced p38 MAPK activation in neutrophils.

(A) Neutrophils were incubated in anti-LacCer IgM T5A7–coated (anti-LacCer IgM) or normal IgM–coated (normal IgM) 10 cm dishes at 37°C for indicated periods. After incubation, lysates were prepared and immunoprecipitated by rabbit anti–p38 MAPK IgG, followed by SDS-PAGE and electroblotting. The membranes were probed with mouse anti–pp38 MAPK IgM (pp38 MAPK). Further, membranes were reprobed with rabbit anti–p38 MAPK IgG (p38 MAPK). (B) Phosphorylation of p38 MAPK was calculated by scanning the signals of phosphorylated and recovered p38 MAPK by Scion image. The activity of p38 MAPK is expressed as fold increase compared with that of cells kept on ice (0 minute). Each point shows the mean ± SD of independent 3 experiments.

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