American Society of Hematology

Fig. 11.

$Fig. 11. Reconstitution experiments of anti-LacCer antibodiy-induced superoxide generation. / Neutrophil DIM and the high-density fractions were isolated as described in Figure 3, and each fraction and mixture of the fractions (2 × 107 equivalent cells per milliliter) were incubated with 1 μg/mL anti-LacCer IgM T5A7 (anti-LacCer), 1 μg/mL normal mouse IgM, 0.1 μM arachidonic acid (arachidonic acid), or 0.5% DMSO (DMSO) for 30 minutes at 37°C in the presence or absence of superoxide dismutase. Superoxide production was calculated by measurement of the superoxide dismutase–inhibitable reduction of cytochrome c at 550 nm. Each bar shows the mean ± SD of independent 3 experiments. Anti-LacCer/PP1, the mixtures of DIM and high-density fractions incubated with 1 μg/mL T5A7 in the presence of 1 μM PP1.$

Reconstitution experiments of anti-LacCer antibodiy-induced superoxide generation.

Neutrophil DIM and the high-density fractions were isolated as described in Figure 3, and each fraction and mixture of the fractions (2 × 10⁷ equivalent cells per milliliter) were incubated with 1 μg/mL anti-LacCer IgM T5A7 (anti-LacCer), 1 μg/mL normal mouse IgM, 0.1 μM arachidonic acid (arachidonic acid), or 0.5% DMSO (DMSO) for 30 minutes at 37°C in the presence or absence of superoxide dismutase. Superoxide production was calculated by measurement of the superoxide dismutase–inhibitable reduction of cytochrome c at 550 nm. Each bar shows the mean ± SD of independent 3 experiments. Anti-LacCer/PP1, the mixtures of DIM and high-density fractions incubated with 1 μg/mL T5A7 in the presence of 1 μM PP1.

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