CD8α⁺CD11c⁺ DC deficiency is due to the lack of ICSBP expression within the hemopoietic compartment.

Bone marrow chimeras were made by intravenous injection of a mixture of bone marrow cell suspensions (1:1 ratio, 1 × 10⁶cells/animal in a volume of 0.2 mL) from wild-type mice congenic for the CD45.1 marker and ICSBP^−/− animals, which are CD45.2⁺. Wild-type (A) or ICSBP^−/− (B) hosts were irradiated (9 Gy [900 rads]) prior to bone marrow reconstitution. Eight weeks later, LOD cells were obtained and stained with anti-CD45.2–FITC, anti-CD11c–PE, anti-CD8α–PerCP, and anti-CD45.1–APC. Cells CD11c⁺CD8α⁺ (upper gate and panel) or CD11c⁺CD8α⁻ (lower gate and panel) were analyzed for CD45.1 (wild-type) versus CD45.2 (ICSBP^−/−) expression. The values expressed at the corners of each panel represent the relative frequency of cells within that specific quadrant (n = 3 or 4 animals/group).