Impaired functional maturation of ICSBP^−/−CD8α⁻ DCs in vivo.

Wild-type control and ICSBP^−/− mice were injected intraperitoneally with PBS (0.2 mL/mouse) or 1 μg oligo-CpG DNA orE coli LPS. Six hours later low-density spleen cells were purified and the expression of CD40 (A), CD80 (B), and I-A (C) was analyzed by FACS within the cells of the DC subsets: CD11c⁺CD8α⁺CD4⁻ (DN), CD11c⁺CD8α⁻CD4⁺ (CD4), CD11c⁺CD8α⁺CD4⁻ (CD8) as indicated. Bars represent mean fluorescence intensity (MFI) ± SEM of each marker in each subset analyzed. (D) Representative micrographs of CD11c-stained spleen sections from wild-type (i and ii) or ICSBP^−/− (iii and iv) mice injected with PBS (i and iii) or LPS (ii and iv) as described above. Orginal magnifications, × 200.