![Fig. 1. Pictures with both the normal and abnormal patterns for the break-apart and fusion strategies. / In all cases the clonal PCs are identified by the intense blue fluorescence of the cytoplasm as detected by the cIg-FISH procedure. (A) A PC with the normal configuration for the VH (red signal) and CH probes (green signal). Arrows point to the closely associated pairs of signals indicative of a normal pattern. (B) Segregation (break-apart) of the VH (green) and CH (red) probes consistent with a 14q32 translocation. (C) A cell with fusion signals (arrows) resulting from comigration of the pools of probes at 14q32 (green) and 4p16.3 (red) consistent with a t(4;14)(p16.3;q32). (D) The fusion signal (arrows) resulting from comigration of the pools of probes at 14q32 (green) and 16q23 (red) consistent with a t(14;16)(q32;q23). (× 100 magnification, Leica DMRXA microscope [Wetzlar, Germany]).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/100/4/10.1182_blood.v100.4.1417.h81602001417_1417_1424/6/h81622989001.jpeg?Expires=1735554153&Signature=OTfbybcYsCUF84xq3TkDjgNWcgQpqxtizplDpqe7NCO7LeABW3fSJWBa0T7TvydzFpjIS5xEYhR1PO4a00wrxJRMR~xLiofUY2DmPwT3SSzR4QkEPx2EPQ4eqMGS4aah1q~XxVpv8LzBQrqzRYuFY5pgh5F-EnYSQ~NxMpiZSb7BstPA1bZr7riEUmUNjoYOUxgSvAnTHddiqp-hON60CXWNngf2~qPywlgo-oQX7zz8~1XaKWoauHEQP5I5ltMQ5GG~A2NQxMcPPNHXxvzHDdx04Ou8I7tmiAHncfzYofSIfSGK0r216BVEuCuBAdTO1Q5NVJpKrO3xG51ZiRjFZQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Pictures with both the normal and abnormal patterns for the break-apart and fusion strategies.
In all cases the clonal PCs are identified by the intense blue fluorescence of the cytoplasm as detected by the cIg-FISH procedure. (A) A PC with the normal configuration for the VH (red signal) and CH probes (green signal). Arrows point to the closely associated pairs of signals indicative of a normal pattern. (B) Segregation (break-apart) of the VH (green) and CH (red) probes consistent with a 14q32 translocation. (C) A cell with fusion signals (arrows) resulting from comigration of the pools of probes at 14q32 (green) and 4p16.3 (red) consistent with a t(4;14)(p16.3;q32). (D) The fusion signal (arrows) resulting from comigration of the pools of probes at 14q32 (green) and 16q23 (red) consistent with a t(14;16)(q32;q23). (× 100 magnification, Leica DMRXA microscope [Wetzlar, Germany]).
