Structure of the mouse ferrochelatase locus and exon 10–deleted protein.

(A) Diagram indicates the structure of the native ferrochelatase locus (top) and the targeted ferrochelatase locus (bottom). EcoRI restriction endonuclease sites, which can be used for Southern blot genotyping, are indicated with an E. The position of the gene-specific primers used for PCR genotyping of the targeted locus are indicated by bent arrows. The Pr1 primer sequence is located in the 3′ end of the neomycin cDNA, and the Pr2 primer sequence is located in the 5′ end of exon 11. The Pr3 primer sequence is located in intron 10 in a region that has been deleted in the homologous recombinant. (B) Schematic of the ferrochelatase protein and known functional domains (top). Amino acids conferred by exon 10 and approximate location in the protein (bottom). The underlined glycine is a valine in the human sequence.