RT-PCR analysis demonstrates equivalent levels of wild-type and exon 10 mRNA.

Levels of wild-type and mutant ferrochelatase mRNA in liver and spleen were determined using semiquantitative RT-PCR. Schematic indicates the location of the primers in the ferrochelatase cDNA used for RT-PCR analysis (top). Exon status of each PCR fragment is indicated (bottom). The slowest migrating band observed in the spleen +/− lane is a heteroduplex (HD) consisting of a wild-type DNA strand and an exon 10-deleted DNA strand. Genotypes of mice are indicated by +/+ (wild type) or +/− (heterozygous).