IFN-γ shifts monocyte differentiation from DCs to macrophages.
(A) Monocytes were cultured in medium containing M-CSF (circles) or GM-CSF plus IL-4 (triangles) in the absence (filled symbols) or presence (open symbols) of 25 ng/mL IFN-γ. After 5 days, cells were (right panel) or were not (left panel) stimulated for 24 hours with 20 ng/mL LPS. Cells were then irradiated and used to stimulate allogenic T cells. Results are expressed in counts per minute × 10−3as means ± SDs of quintuplicate values. Results shown are of 1 experiment representative of 3. Results show mean SD of quintuplicate values of 1 experiment representative of 3. (B) Monocytes were cultured in GM-CSF plus IL-4 in the absence (left) or presence of 25 ng/mL IFN-γ (right). After 5 days, cells were (top) or were not (bottom) stimulated for 24 hours with 20 ng/mL LPS and observed by microscopy (original magnification, × 100).
