Fig. 3.
IFN-γ enhances M-CSF and IL-6 mRNA expression by monocytes in GM-CSF plus IL-4.
Freshly isolated monocytes were not (none) or were cultured in GM-CSF plus IL-4 in the absence or presence of 25 ng/mL IFN-γ. After 2, 4, 8, and 16 hours, the expression of the mRNA encoding M-CSF, IL-6, IL-10, CD115, and GAPDH was analyzed by RT-PCR.