Results of FCM-WASP of lymphocytes and monocytes.

(A) Density plot shows forward- and side-scatter data from PBMCs. Lymphocytes (G1 gate) and monocytes (G2 gate) are gated according to the values of these variables. (B) FCM-WASP with a 3-decade log scale. The x-axis represents WASP expression; the y-axis represents cell numbers. The open histogram indicates isotype-control staining; the solid histogram indicates specific staining of WASP. Shown is WASP expression in lymphocytes and monocytes from a healthy control (top), a WAS patient with MC status after HST (middle; data from patient 2 on day 360 after HST), and a WAS patient (patient 12) before HST (bottom).