Migration of immature and mature MoDCs toward proinflammatory and CCR7-binding chemokines.

Immature MoDCs (GM + IL-4) or MoDCs stimulated with the indicated stimuli for 2 days were examined for their migratory capacity toward either CCL19 (MIP-3β) or CXCL12 (SDF-1α) in transwell assays. (A) Dose titration of CCL19 in transwell assay and comparison of migratory function of immature MoDCs and MoDCs matured with TNF-α + IFN-α + PGE₂. (B) Migration toward CCL19 (30 ng/mL) by immature MoDCs or MoDCs stimulated with either TNF-α + IFN-α + PGE₂ or CD40L (1 μg/mL) or intact E coli (1 × 10⁶). Data are representative of 5 separate experiments. (C) Migration toward either CCL19 or CXCL12 (50 ng/mL) by immature MoDCs or MoDCs stimulated with either TNF-α + IFN-α + PGE₂ or CD40L (1 μg/mL) or CD40L + PGE₂. Data are representative of 5 separate experiments. (D) Migration toward CCL19 by immature MoDCs or MoDCs stimulated with either TNF-α + IFN-α + PGE₂ or CD40L or CD40L + PGE₂ or intact E coli or intactE coli + PGE₂. Data are representative of at least 5 separate experiments.