Regulation of expression of CCR7 and CXCR4 chemokine receptors.

Immature MoDCs (GM + IL-4) or MoDCs stimulated with the indicated stimuli for 3 days were examined for expression of chemokine receptors by FACS and by quantitative real time PCR (qRT-PCR). (A) Analysis of CXCR4 expression on immature MoDCs or MoDCs stimulated with either TNF-α + IFN-α + PGE₂ or CD40L (1 μg/mL). Data are representative of 3 separate experiments. (B) Analysis of CCR7 expression on immature MoDCs or MoDCs stimulated with either TNF-α + IFN-α + PGE₂ or CD40L (1 μg/mL). Data are representative of 4 separate experiments. (C) Analysis of CCR7 mRNA levels by qRT-PCR on immature MoDCs or MoDCs stimulated with either TNF-α + IFN-α + PGE₂ or CD40L or CD40L + PGE₂ or intact E coli or intactE coli + PGE₂. Data represent the mean ± SEM of 4 separate donors.