Modulation of IL-12p70 production in MoDCs by various stimuli.
On day 7, immature MoDCs were pooled and adjusted to 1 × 105 cells per mL. MoDCs were activated for 3 days with the indicated stimuli, and cytokine ELISAs were performed on culture SN. (A) Secretion of IL-12p70 by immature MoDCs and MoDCs stimulated with either TNF-α + IFN-α + PGE2or CD40L (1 μg/mL) or intact E coli(1 × 106/mL). Data represent the means ± SEM of 7 different donors. (B) PGE2 or forskolin inhibits CD40L orE coli–mediated IL-12p70 secretion. IL-12p70 secretion by immature MoDCs or MoDCs stimulated with either TNF-α + IFN-α ± PGE2 or ± forskolin (1 μM) or CD40L ± PGE2 or ± forskolin (1 μM) or E coli ± PGE2 or ± forskolin (1 μM). Data represent the mean ± SEM of triplicate wells.
