Polyclonal antibody to the longer limitin protein.

(A) 293T cells transfected with the HT/pEFBOSX or Limitin-HT/pEFBOSX plasmids were stained with the indicated antibodies and their immunofluorescent intensities analyzed by flow cytometry. (B) Recombinant limitin, IFN-α, IFN-β, and IFN-γ (50 ng/lane) were subjected to Western blot probed with anti-limitin. (C) 293T cells were transfected with the indicated plasmids. Their supernatants were collected after 3-day cultures and subjected to Western blot probed with antilimitin. (D) The supernatants of the indicated cells were collected after 5 days of culture and subjected to Western blot probed with antilimitin. All panels show 1 of 2 independent experiments.