Limitin mRNA distribution in mice.

(A-C) Total RNAs (2.5 μg) isolated from the indicated tissues (A), the thymocytes of the indicated ages of mice (B), and the subpopulations of lymph node cells purified using immunomagnetic beads conjugated with the indicated antibodies (C) were subjected to RT-PCR. The amplified products were electrophoresed through 1% agarose gels containing ethidium bromide. (D) cDNA from the sorted naive (CD4⁺CD45RB^high) and memory (CD4⁺CD45RB^low) T cells of lymph node cells were obtained using the Cells-to-cDNA kit (Ambion, Austin, TX) and subjected to PCR. (E) Balb/c mice were injected lipopolysaccharide (400 μg/mouse) or herpes simplex virus (5 × 10³plaque-forming units per mouse) intraperitoneally. After 24 hours of injection, their lymph node cells were collected and used for RNA preparation, followed by the indicated cycles of PCR. All the panels show 1 of 2 independent experiments that gave similar results.