Correction of aberrant splicing by lentiviral plasmids in β-globin IVS2 mutant HeLa cells.

Total RNA from HeLa β-globin IVS2 mutant cells transiently transfected with 0.05 to 1.0 μg pTKU7.623r lentiviral construct was analyzed by RT-PCR. (A) β-Globin IVS2-654, (B) β-globin IVS2-705, and (C) β-globin IVS2-745 cells. Lane 1, untreated cells; lanes 2 to 5, cells transfected with pTKU6.623r; lane 6, (N) RNA from normal human blood.