Fig. 6.
Production of human erythroid cells in liquid expansion cultures.
Marrow cells obtained from mice engrafted for 6 weeks with transduced human fetal liver cells were placed in liquid expansion cultures and maintained for 3 weeks as described in “Materials and methods” at the end of which they were stained with antihuman glycophorin A (right panel) and Ter 119 (left panel) to detect any amplified murine erythroid cells. Shown here is the FACS dot plot obtained from a representative experiment in which the cells used to initiate the culture were from an engrafted NOD/SCID-β2m−/− mouse.
