Fig. 1.
Fig. 1. Procedure for the development of mAbs specific for. / P falciparum IE surface antigens. B cells of 24- to 48-hour-old Balb/c mice were rendered tolerant to nEs and CHO-745 by subcutaneous injection of 2 × 109 cells. Mice were given a boost 21 days later by intraperitoneal injection of 5 × 106 nEs or 5 × 105 nCHO-745 cells. Tolerant animals were identified by L-IFA with a 1:10 dilution of serum, 21 days after the boost (day 42). Animals given no IF or only weak IF were then immunized and boosted with 5 × 106mIECSA or 5 × 105 CHO–DBL-γ3 transfectant. Spleen cells from animals that responded (day 74) to mIECSA or DBL-γ3 were then used to raise mAbs by fusing these cells with P3U1 cells. The specific antiparasite surface immune responses were assessed by L-IFA.

Procedure for the development of mAbs specific for

P falciparum IE surface antigens. B cells of 24- to 48-hour-old Balb/c mice were rendered tolerant to nEs and CHO-745 by subcutaneous injection of 2 × 109 cells. Mice were given a boost 21 days later by intraperitoneal injection of 5 × 106 nEs or 5 × 105 nCHO-745 cells. Tolerant animals were identified by L-IFA with a 1:10 dilution of serum, 21 days after the boost (day 42). Animals given no IF or only weak IF were then immunized and boosted with 5 × 106mIECSA or 5 × 105 CHO–DBL-γ3 transfectant. Spleen cells from animals that responded (day 74) to mIECSA or DBL-γ3 were then used to raise mAbs by fusing these cells with P3U1 cells. The specific antiparasite surface immune responses were assessed by L-IFA.

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