DGGE-based detection of ATM variants and allelic loss.

(A) DGGE analysis of a region spanning ATM exon 65, showing aberrant bands in samples DLBCL-22 and DLBCL-101. In the latter case, the mutant band is more intense than the wild-type band, suggesting loss of the wild-type allele. (B) Direct sequence analysis of exon 65, showing the R3008H (9023G>A) mutation in DLBCL-22. (C) Detection of anATM deletion in DLBCL-26 as manifested by loss of one of the alleles of the 8850 + 60G/A marker by DGGE analysis. Pixel intensity and gel distance are in arbitrary units.