Rats that spontaneously reject mM-CSF T9-C2 cells are immune to intracranial T9 gliomas.
Rats were immunized for 2 weeks with 3×105 living T9-C2 cells and then implanted with 104 T9 cells intracranially (A). Data are also shown from rats that were immunized with living T9-C2 cells for 2 weeks, after which they were killed. Splenocytes were then removed and adoptively transferred into naive rats that were implanted with 104 T9 cells intracranially. (B) Rats were injected with 3×105 T9-C2 cells that were untreated (n = 20), x-irradiated with 10G (n = 7), treated with mitomycin-C (n = 6), or killed by freeze-thawing (n = 9). As a comparison, T9 cells treated with mitomycin-C were injected into 9 rats. After 2 weeks, the various immunized rats were implanted with 104 T9 cells intracranially. The rats that had been immunized with the living T9-C2 cells were significantly different (P < .001) from all other groups.
