American Society of Hematology

Fig. 3.

$Fig. 3. Immunoblot analysis of IL-7R in HS-27a, LTCs, and PBMCs. / (A) Total protein extracts of HS-27a, LTCs, and PBMCs were analyzed for IL-7R by Western blot. The 30-kd form, which bound to the anti–IL-7R-IC antibodies, was detected in both HS-27a and primary stromal cell extracts (LTCs) but was almost absent in the PBMC extract. Comparable results were obtained from 4 different cell preparations. (B) Subcellular fractions (26 μg/lane) of HS-27a cells were analyzed by Western blot. Lane 1 represents the cytosol fraction; lane 2, the membrane fraction; and lane 3, the insoluble fraction. The full-length form of IL-7R (90 kd) was found in the membrane fraction, and the 30-kd form was exclusively found in the insoluble fraction of HS-27a cells. This is in keeping with the immunocytochemistry data that show staining localized to the nucleus.$

Immunoblot analysis of IL-7R in HS-27a, LTCs, and PBMCs.

(A) Total protein extracts of HS-27a, LTCs, and PBMCs were analyzed for IL-7R by Western blot. The 30-kd form, which bound to the anti–IL-7R-IC antibodies, was detected in both HS-27a and primary stromal cell extracts (LTCs) but was almost absent in the PBMC extract. Comparable results were obtained from 4 different cell preparations. (B) Subcellular fractions (26 μg/lane) of HS-27a cells were analyzed by Western blot. Lane 1 represents the cytosol fraction; lane 2, the membrane fraction; and lane 3, the insoluble fraction. The full-length form of IL-7R (90 kd) was found in the membrane fraction, and the 30-kd form was exclusively found in the insoluble fraction of HS-27a cells. This is in keeping with the immunocytochemistry data that show staining localized to the nucleus.

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