Immunofluorescence localization of intracellular IL-7R.

HS-27a cells were fixed with methanol and incubated with the anti–IL7R-IC antibodies (A) or control immunoglobulins (B). The bound antibodies were detected with Cy5-conjugated secondary antibodies (red). Nuclei of the cells were counterstained with DAPI (blue), and 2-color fluorescence images were captured with a deconvolution fluorescence microscope. The images of a nucleus stained with the anti–IL7R-IC antibodies are reconstructed into a 3-dimensional image and rotated by 45° 4 times as shown in panel C. This clearly shows localization of the intracellular receptor in the nucleus. Original magnification × 400.