Cytokine production of IL-7–stimulated HS-27a cells.

(A) Concentrations of the cytokines IL-6, SDF-1α, IL-1β, and macrophage inhibitory protein 1-α (MIP-1α) in conditioned media were determined after 6 days of culture without (open bars) or with 100 ng/mL IL-7 (hatched bars) by ELISA. Each bar represents the average ± SD for 4 samples. (B) Northern blot analysis to measure the IL-6 (upper gel) and actin (lower gel) mRNA levels in IL-7 stimulated (+) or control (−) HS-27a cells at different time points. Each lane contained total RNA (10 μg/lane) blotted onto a nylon membrane. The membrane was probed with a P³²-labeled IL-6 probe (upper gel). The bound probe was stripped off, and the membrane was reprobed with a P³²-labeled actin probe (lower gel). (C) The blots were quantitatively scanned, and the ratio of IL-6 RNA to actin RNA was calculated. The IL-7–stimulated and control cells are represented by solid and dashed lines, respectively.