Fig. 3.
Suppression of IL-2–induced PI 3-kinase activation by inhibiting Syk.
IL-2–starved NK92 cells, treated with 25 μM piceatannol or infected with recombinant vaccinia virus encoding SykT or CD56 irrelevant control gene, were stimulated with IL-2 (100 U/mL) for 5 minutes at 37°C. Cells were then analyzed for PI 3-kinase activity by in vitro kinase assay and thin-layer chromatography analysis with PI3,4P2 as the substrate. Results are representative of 1 of 3 independent experiments.
