Cytotoxic activity of untreated and irradiated NK-92-scFv(FRP5)-ζ against primary and established human breast cancer cells.

Breast cancer cells were collected from a pleural effusion of a patient suffering from metastatic breast cancer. ErbB2 expression on the surface of primary cancer cells was confirmed by FACS analysis (A). Primary (B) and established SKBR3 breast cancer cells (C) were incubated with NK-92-scFv(FRP5)-ζ (filled bars), NK-92-scFv(FRP5)-ζ irradiated with a dose of 10 Gy (hatched bars), or parental NK-92 cells (open bars) at an E/T ratio of 10:1 for 2 hours. Specific lysis was determined in europium-release assays as described in the legend of Figure 2.