Rapamycin inhibits proliferation of B-CLL cells stimulated with CpG-ODN DSP30 and IL-2.
B-CLL cells were cultured at 106 cells/mL in medium alone or in the presence of 1 μm DSP30 and 100U/mL IL-2 with or without the indicated concentrations of rapamycin. Thymidine incorporation was analyzed after 72 hours of culture, and results are shown as means ± SEMs of triplicates. Panel A shows 1 representative experiment (means ± SEM of triplicate cultures) of 3 performed. Inhibition of thymidine incorporation in stimulated B-CLL cells (DSP30 and IL-2) from 13 patients by rapamycin at 50 ng/mL is shown in panel B as means ± SEMs. In 3 additional experiments, rapamycin was either added at the indicated concentrations from the beginning of the culture (▪) or after 24 hours of stimulation (■). Thymidine incorporation was measured after 72 hours as described in “Patients, materials, and methods.” Panel C shows one representative experiment (means ± SEMs of triplicates).
