Fig. 3.
p70S6k phosphorylation in B-CLL cells is inhibited by rapamycin.
(A) p70S6k expression and phosphorylation at threonin 389 (p-p70s6k) during 24 hours of activation with DSP30 and IL-2 were analyzed using specific antibodies. Cellular extracts of 293 cells cultured in the absence of or in the presence of serum were used as a positive (pc) and negative control (nc), respectively. (B) The same experiment was performed in the presence of 50 ng/mL rapamycin. Similar results were obtained in 2 additional experiments (data not shown).
