Fig. 7.
![Fig. 7. Rapamycin does not affect apoptosis in cycling or resting B-CLL cells. / (A) Annexin V and PI staining were performed in B-CLL cells after 48 or 72 hours of culture in the presence of medium alone or DSP30 and IL-2 (stimulated/activated[stim]) with or without rapamycin (Rap). Viable cells were annexin- and PI-negative. Results were obtained from 5 different samples and are presented as means ± SEMs. (B) For TUNEL staining, cells were cultured for 72 hours using the same conditions as described above. One additional independent experiment gave similar results.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/101/1/10.1182_blood-2002-01-0189/6/h80133615007.jpeg?Expires=1735577650&Signature=VVYH4jOTP1IXLaJ-lkSorzKK72hXQyBNN5PIvSLsgVL6Fh0~zhkvXjtuv3d0nBRyM0qL8ze0DsYCWiRZYtFdM0bMmTgjrOnOS0JTUaG0FjhcOn7ZTbl9ZgHosbw9qexqQOiDKLvl6i5Oqe~hy-nOxaXePxqLl1kZhjzm9XjMKCY48e5r7VUIqRe~6kl5FuynAjNw-fEyxmgFisdFp9ubvYwHIsOCAsSOWOVOPmmVPTExjf1jOJ~EyH8ktXx~Rzwfd5nZL5wkfujvYju5nuDE9TmGi2kKhA1Shv3~7YE4SjnpJg3EGGp0ndlWPLTVIwUnMe5a5vFzw0n3eQpn5DW9JA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Rapamycin does not affect apoptosis in cycling or resting B-CLL cells.
(A) Annexin V and PI staining were performed in B-CLL cells after 48 or 72 hours of culture in the presence of medium alone or DSP30 and IL-2 (stimulated/activated[stim]) with or without rapamycin (Rap). Viable cells were annexin- and PI-negative. Results were obtained from 5 different samples and are presented as means ± SEMs. (B) For TUNEL staining, cells were cultured for 72 hours using the same conditions as described above. One additional independent experiment gave similar results.
