Survival of transendothelial migrated and nonmigrated cells without growth factors.

PBMCs were allowed to transmigrate overnight across HUVEC monolayers. After 72 hours of culture, nonmigrated and migrated cells were stained with FITC–annexin V (A) or with 50 μg/mL PI solution (B-C). PI staining of freshly isolated PBMCs (D) and of cells treated for 24 hours with 0.5 μg/mL of the anti–Fas mAb CH-11 (E) was also performed. Samples were then analyzed on a FACSort and results plotted as a percentage of annexin V⁺ cells (A) or as log red fluorescence intensity (au) for PI, versus number of cells (B, migrated cells; C, nonmigrated cells; D, freshly isolated PBMCs; E, CH-11–treated cells). Arrows in panels B-E indicate DNA content less than 2n (apoptotic cells). One representative experiment of 6 is shown.