Kinetics of starvation-induced apoptosis after transendothelial migration.

Peripheral blood adherent mononuclear cells were allowed to transmigrate overnight across HUVEC monolayers. Nonmigrated (○) and migrated (●) cells were cultured in the absence of growth factors (A-B), or with GM-CSF (C), collected at the indicated time points, and stained with FITC–annexin V and PI. Samples were then analyzed on a FACSort and results plotted as a percentage of annexin V⁺(A) or percentage of apoptotic (DNA content < 2n-diploid) cells (B-C). Mean values ± SDs from 6 different experiments are shown.