Viability of cells migrated through endothelium.

Peripheral blood CD14⁺ cells were allowed to migrate across HUVEC monolayers. (A) Migrated cells. (B) Nonmigrated cells. (C) Cells migrated through the naked filter without endothelium. Cells were submitted to triple staining with HO, AO, and PI and analyzed in fluorescence microscopy. Notice in panel A the prevalence of green living cells on red and yellow apoptotic cells. Nonmigrated cells (B) appear mostly as apoptotic-necrotizing cells (yellow nuclei) or late apoptotic–necrotizing cells (red nuclei). Conversely, only orange-stained cellular debris are detectable when cells passed through the naked filter (C). One representative experiment of 4 is shown; original magnification × 200 for all panels.