RPS19 protein expression during terminal erythroid differentiation.

(A) We tested the sensitivity of our antibody raised in chicken-against-mouse recombinant His-tagged RPS19 protein to detect the recombinant protein. The antimouse RPS19 was able to readily detect 100 ng of recombinant RPS19 protein. A molecular weight standard (MWs) was loaded in the first lane. (B) RPS19 protein expression in FAV cells during terminal erythroid differentiation. As with RPS19 mRNA expression, RPS19 protein expression also decreased during terminal erythroid differentiation. The arrow indicates the location of the RPS19 protein in the gel. The expression of actin during the same time course of terminal erythroid differentiation is shown in the right panel. In contrast to the marked reduction in RPS19 protein expression during the late stages of erythropoiesis, the level of actin expression showed little change. MWs was loaded in the first lane, and the positions of the molecular weights are indicated above lanes.