Figure 2.
BCR triggering leads to rapid up-regulation of TLR9 and TLR10 in naive B cells. (A) Naive (i), IgM memory (ii), and switch memory (iii) B cells were isolated as in Figure 1 and cultured in the presence or absence of CpG 2006 and F(ab′)2 fragments of goat antihuman Ig as a BCR trigger. At different time points cells were harvested and the expression of TLR9 was measured by real-time PCR. The data are normalized to the expression of β-actin. Data are the mean ± SD of 4 experiments. ○ indicates medium; ▵, CpG; □, anti-Ig; and ⋄, CpG + anti-Ig. (B) Kinetics of TLR9 and TLR10 up-regulation in naive B cells determined by semiquantitative PCR. One representative experiment of 4.
