TC-A induces the up-regulation of HSP70 in EHEB cells and native B-CLL cells. EHEB cells (left) or primary B-CLL cells (right) were left untreated or treated with 0.5 μM, 1 μM, or 2.5 μM TC-A for 24 hours. Subsequently, up-regulation of HSP70 was detected by immunoblot analysis. As internal loading control for equal amounts of protein, α-tubulin was used. One representative result from at least 2 independent experiments is shown.