Figure 3.
Time course of protein tyrosine phosphorylation induced by 2B-rVWF. Platelets (2.8 × 108/mL) were either mixed with buffer (–) or with 2B-rVWF at 0.7 μg/mL (+) in the absence (0 s–1) or in the presence (4000 s–1) of high shear rate. Proteins of the lysates were separated on a 8% polyacrylamide gel, transferred to a nitrocellulose membrane, and blotted with antiphosphotyrosine (A) or anti-pFAK/FAK antibody (B), using either anti–phospho-FAK or anti-FAK. On the right side, resting or thrombin-stimulated platelets are shown. Data are representative of 3 experiments.
