Figure 4.
Effect of various concentrations of 2B-rVWF or WT-rVWF on SIPA and p125FAK phosphorylation. Washed platelets (2.8 × 108/mL) were exposed to 4000 s–1 for 4.5 minutes at 37°C in the presence of various concentrations of 2B-rVWF (A; □, ▪) or WT-rVWF (B; ▵, ▴). An aliquot was taken for quantitation of SIPA expressed as % DSP (left vertical axis; □, ▵). Corresponding lysates were analyzed by Western blotting with anti–phospho-FAK antibody, and autoradiographs were scanned with a laser densitometer and expressed as OD arbitrary units (AU) (right vertical axis; ▪, ▴). Results are representative of 2 experiments.
