hTERT-transduced cell cultures contain more cells that express bcl-2 than cells expressing active caspase-3. T-cell cultures described in Figure 5 were stained intracellularly with anti–Bcl-2–FITC and antiactive caspase-3–PE mAb at day 15, 22, 29, 43, 50, and 57 during the cultures. Graphs show the double staining plots and the percentage of single- or double-positive cells in the culture of untransduced or hTERT-transduced MoT-72 cells. Top left plot shows the incubation with isotype control antibodies. In hTERT-transduced cultures an accumulation of Bcl-2–positive cells was seen, whereas the balance between Bcl-2–positive cells and cells expressing active caspase-3 was reversed in untransduced cultures. Analysis of the culture of untransduced and hTERT-transduced AKR103 cells gave similar results.