Sorting procedure to enrich for CD79a⁺CD19^– cells at day 14. (A) CD34⁺CD10^–CD19^– progenitors sorted from fresh or thawed cord bloods were incubated on MS-5 stromal cells with SCF, IL-2, and IL-15. After 2 weeks, all nonadherent and adherent nucleated cells from 10 to 14 wells were collected and labeled with anti–CDw127-PE, a mixture of anti-CD11b and anti–CD36-FITC-Cy5, and anti–CD19-PE-Cy5. Cells positive for the CD11b⁺ and CD36⁺ markers represented 50% of the population, and CDw127⁺ represented 10%, half of which coexpressed CD19. CDw127⁺CD11b^–CD36^–CD19^– cells were sorted as detailed in “Materials and methods.” (B) Sorted cells were reanalyzed after labeling with anti-CD79a and CDw127 to evaluate their purity and morphologic profile. The panel on the right shows that more than 85% of the cells were CD79a⁺ and had a very low SSC and heterogeneous FSC values. All CD79a^– contaminants fell into gate R2 indicated in the left panel.