Expression of B-cell transcripts in CD79a⁺-enriched CDw127⁺CD19^– cells. Four populations of flow cytometry–sorted cells were analyzed by RT-PCR. Lane A: fresh CD34⁺CD10^–CD19^– input cord blood cells; lane B, cultured CDw127⁺CD11b^–CD36^–CD19^– cells, including 85% CD79a⁺, sorted twice at day 14; lane C, cultured CD19⁺ B cells sorted at day 14; lane D, cultured CD19⁺ B cells sorted at day 21. Cells used in lanes A-D were double-sorted to ensure more than 98% purity. The preparation of cDNAs and procedure used for the PCR reaction are described in “Materials and methods.” The cDNA input for each population was normalized to obtain equivalent signals with the S14 gene. All lanes were run in the same gel. Data shown are from 1 of 3 similar experiments with similar results.