Immunophenotype of differentiated cells generated by CD79a-enriched CDw127⁺ cells. CD79a⁺-enriched CDw127⁺CD11b^–CD36^–CD19^– cells generated by CD34⁺CD10^–CD19^– cord blood cells were sorted at day 14 and incubated 14 days on MS-5 stromal cells in culture conditions that favor B and NK lymphoid differentiation (middle column, top panel) or macrophage differentiation (middle column, middle panel). After 14 additional days in these conditions, cells were stained with lineage-specific antibodies and analyzed by flow cytometry as illustrated. FSC/SSC profiles of CD19⁺, CD56⁺, and CD14⁺ cells are indicated on the right panels. CD79a⁺-enriched CDw127⁺CD11b^–CD36^–CD19^– cells were also grown for 35 days in FTOC organotypic culture (middle column, bottom panel). After the culture, cells collected by mechanical dissociation were labeled with MoAbs against CD4, CD8, and CD1a.