Figure 8.
PCR analysis of DJH and VDJH gene rearrangements on DNA from differentiated CD19+, CD56+, and CD14+ cells generated by CD79a+-enriched CDw127+CD11b–CD36–CD19– cells. (A) BLIN1 and CD34–CD19+ were used as controls as described in the legend of Figure 4. CD19+ B cells, CD56+ NK cells, and CD14+ macrophages were obtained from cultured CD79a+-enriched CDw127+CD11b–CD36–CD19– cells and sorted twice at day 28 (> 98% purity). DNA was extracted from 20 000 cells, and PCR was performed as described in “Materials and methods.” (B) Results of PCR performed on serial dilutions of DNA extracted from CD19+ cells purified at day 28 as described in panel A.