TSH-induced secretion of TNF-α by CD11b⁺and CD11b⁻ BM cells.

MACS-sorted cells were cultured with graded doses of human TSH as described in “Materials and methods.” Note the significant increase in TNF-α secretion in TSH-stimulated versus unstimulated CD11b⁻ cells and the predominant TSHR⁺ cells population (P < .05) (Figure 5). Among CD11b⁺ cells there was no significant difference in TNF-α secretion of TSH-stimulated cells compared to unstimulated cells. TNF-α production by LPS-stimulated cultures is shown for comparison. Data are mean values ± SEMs of 2 to 5 samples per group.