Figure 1.
GPVI and GPIaIIa surface expression in homozygous “aa” and “bb” donors. Platelets were analyzed in flow cytometry assay with GPVI-specific scFv 1C3 (A) and scFv 10B12 (B) and GPIaIIa-specific moAb NBS-P16 (D). The levels are presented as MFI, with binding of the negative control antibody (moAb 9E10) for each sample subtracted (MFI Corr). Panel C shows the correlation between the fluorescence produced by the 2 GPVI-specific scFvs.